Se diseñaron los biorreactores, utilizando principios de similitud; para la producción del alcohol etílico, se diseñó un biorreactor del tipo Lecho Empacado . Inmovilización de levaduras en residuos lignocelulósicos para la producción de etanol en biorreactor de lecho empacado. En las fermentaciones realizadas en los biorreactores de lecho empacado con el biocatalizador (soporte + levaduras), se logró obtener un aumento en la.

Author: Mizilkree Shashakar
Country: Bahamas
Language: English (Spanish)
Genre: Technology
Published (Last): 22 September 2016
Pages: 89
PDF File Size: 9.15 Mb
ePub File Size: 14.97 Mb
ISBN: 748-8-12276-968-1
Downloads: 32152
Price: Free* [*Free Regsitration Required]
Uploader: Mikarg

Yeast immobilization in lignocellulosic wastes for ethanol production in packed bed bioreactor

Figure 3 shows the growth of microorganisms in petri dishes containing nutrient agar and pieces of non-sterile materials; however, there is no growth in the petri dishes that were placed in sterile lignocellulosic residues.

At the output of the reactor we obtained concentrations from We have also established that the glucose syrup obtained from the enzymatic hydrolysis of cassava flour can be used as an alternative substrate in alcoholic fermentations. This demonstrates that if further investigations are carried out to establish the best times for the immobilization process and the best fermentation conditions, high yields of ethanol production can be reached by using immobilized cells in the carriers studied.

Analytical Chemistry, 31 3pp.

Un biorreactor Novela de alta densidad de cultivo de diversas comunidades microbianas

For the fermentation performed with glucose, the productivity reached was Show full item record. These immobilization mechanisms are not only dependent of the surface characteristics of the carriers, they depend on cell surface characteristics, the carrier used and the operational conditions under which the immobilization bioreactor carried out flow, temperature, etc.


High oil prices and decrease of world stock, in addition to the concerns about the continuous deterioration of the environment, have given a strong push to the biorreeactor for alternative energy sources in recent years, particularly the development of liquid biofuels to replace oil and its derivatives, and reduce the negative impact on the atmosphere caused ed burning fossil fuels.

Ajuste de mezcla de velocidad en un entorno necesario para establecer una frontera clara algas dentro del reactor Figura 2 ; la zona de la biomasa de algas debe ser de aproximadamente cm de altura.

The response variables evaluated in this study were the reduced sugars consumption, lefho ethanol production. Figure 4 presents the results of the images obtained from scanning electron micrographs SEM.

The continuous processes that use immobilized cells show several advantages with respect to continuous processes with free cells biorresctor.

A Cole Parmer peristaltic pump model was used, operating at a flow of 0.

The baseline immobilization was performed by following the yeast immobilization biorrewctor. The production of ethanol obtained at the output of the reactor was Permita que las algas se asiente dejando las muestras de agua en reposo durante 24 horas. We evaluated four different waste lignocellulosic materials: Biomass quantification Protocol dry weight modified empacaco After completion ofthe 12 hours of immobilization baseline, the biomass quantification process developed was as follows: The concentration of biomass was determined by optic density at nm using a curve of dry weight.

Revista Facultad de Ingeniería

To determine the size range of the carriers, we considered biorractor bioreactor used and mass transfer criteria for packed bed columns. Consulte la tabla de Materiales para las especificaciones de tubos adicionales. Anaerobic conditions must be ensured as well, so that the substrate consumption is oriented to the production of ethanol and not to increase biomass quantity during fermentation.


Instale los cabezales de bomba en las unidades empacadp bombeo con la provi tornillosded con las unidades de bombeo.

Modelado de reactores de lecho empacado en COMSOL®

Consultado el 10 de Julio de To establish immobilization capacity on each of the materials and determine the effect of size and flow rate in the process, we standardized the biomass quantification methodology on the carriers. You will only be able to see the first 20 seconds.

The ethanol concentration was determined by HPLC, by analyzing samples of the effluent of the reactors. The experimental design analysis using Design Expert software allowed us to establish that there is no significant statistical difference between treatments. Immobilization yeasts cells, bioethanol, biofuels, lignocellulosic carriers, continuous fermentation.

The medium for inoculum and fermentation is biorrdactor of: Analytic methods The total sugar quantification was performed by the DNS Dinitrosalicylic acid colorimetric method. Figure 8 shows the cells immobilized on each of the four evaluated carriers. Calcular la densidad de la biomasa dentro de la muestra concentrada.

The cellulosic materials are regenerable, reusable, sterilizable on heat, biologically and chemically stable under different fermentation conditions and with adequate mechanical resistance [18]. To establish which of these mechanisms were involved in immobilization on the lignocellulosic materials used, it is necessary to study each of these characteristics.